The enzyme argininosuccinate synthetase catalyzes the formation of argininosucci
The enzyme argininosuccinate synthetase catalyzes the formation of argininosuccinate from citrulline and aspartate in an ATP dependent manner ( reaction shown below):
Citrulline + ATP+L-Asp→ Argininosuccinate +AMP+PPi
In a test tube, the enzyme-catalyzed reaction is readily reversible , yet in a cell the enzyme-catalyzed reaction is highly irreversible.
Provide a detailed thermodynamic/ chemical explanation as to why this reaction is largely irreversible in a cell but not in a test tube.